Multiple Drug Saliva Test - 7 in 1 single step test
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Multiple Drug Saliva Test - 7 in 1 single step test
This test is a simple, quick, reliable and highly accurate test which detects for the presence of drugs of abuse in saliva. You will receive accurate results within seconds. Tests for Amphetamine, Cocaine, Methamphetamine, Methadone, Opiates, Marijuana, and Phencyclidine
Principle of the Test
The Multi Saliva Drug Test (Oral Fluid) is an immunoassay based on the principle of competitive binding. Drugs that may be present in the oral fluid specimen compete against their respective drug conjugate for binding sites on their specific antibody.
During testing, a portion of the oral fluid specimen migrates upward by capillary action. A drug, if present in the oral fluid specimen below its cut-off concentration, will not saturate the binding sites of its specific antibody. The antibody will then react with the drug-protein conjugate and a visible coloured line will show up in the test line region of the specific drug strip. The presence of drug above the cut-off concentration in the oral fluid specimen will saturate all the binding sites of the antibody. Therefore, the coloured line will not form in the test line region.
A drug-positive oral fluid specimen will not generate a coloured line in the specific test line region of the strip because of drug competition, while a drug-negative oral fluid specimen will generate a line in the test line region because of the absence of drug competition.
To serve as a procedural control, a coloured line will always appear at the control line region, indicating that proper volume of specimen has been added and membrane wicking has occurred.
Frequently Asked Questions (FAQ’s)
1. Q: How is the sample collected?
A. The donor actively swabs the inside of the mouth and the top of the tongue. As soon as the sponge softens slightly, gently press the sponge between the tongue and teeth to ensure complete saturation (about 3 minutes).
1. Q: What drugs does the device detect and at what cut-offs?
A: A combination of Amphetamine, Cocaine, Methamphetamine, Methadone, Opiates, Marijuana, and Phencyclidine.
|Abbreviated name on test||Proper name||Other names||Approximate detectable time it remains in the Saliva after use.|
|AMP||Amphetamines||Speed, amph or whizz.||10 MIN - 72 HOURS|
|BZO||Benzodiazepines||Valium, Tranks.||10 MIN - 48 HOURS|
|COC||Cocaine||Coke, crack or charlie.||10 MIN - 24 HOURS|
|MET||Methamphetamines||Glass, ice or meth.||UP TO 14 HOURS|
|MTD||Methadone||Dolly, Red Rock||10 MIN - 72 HOURS|
|OPI||Opiates||Heroin, smack or gear.||UP TO 2 DAYS|
|THC||Marijuana||Cannabis, puff, spliff or hash.||1 HOUR - SEVERAL DAYS|
2. Q: What is the detection window compared to other drug testing methods?
A: Saliva and blood have similar detection windows. Testing saliva/blood will detect drug use faster than testing urine. Saliva/blood may detect drug ingestion immediately while drug detection in urine may take 6-8 hours post ingestion.
3. Q: What is a lateral flow device?
A: The oral fluids test is based on competitive binding. Drugs that may be present in the oral fluid specimen compete against their respective drug conjugate for binding sites on their specific antibody. During testing, a portion of the oral fluid sample migrates across the membrane. If no drug is present above the cut-off, the sample will not saturate the binding sites of its specific antibody. The antibody will then react with the drug-protein conjugate and a visible coloured line will show up in the test line region. The presence of a drug above the cut-off concentration will saturate all the binding sites of the antibody and a line will not form in the test region. The lateral flow is the migration and competition across the membrane.
4. Q: Does the test quantify the concentration of drugs present in the oral fluids sample?
A: A positive test result does not indicate the concentration of drug in the sample. All positive results are presumptive and should be confirmed by an alternate method (e.g. GC/MS or GC/MS/MS). Negative results may not necessarily indicate a drug-free sample. Drug may be present in the sample below the cutoff level of the assay.
7 Drug Saliva Test Procedure:
You can read negative results as soon as the lines form, but for positive results please wait the full 10-minute development time. Please note the drug type code on the test case and read against the code for each drug type.
Read the results of each individually and independent of one another. The top line in each result (line nearest the top of the oral cube case) is the control line and the lower line (closest to base of cube case) is the test line. The control line means the test has worked correctly. If there is no upper line then the test is invalid as shown below. This means that there has been insufficient specimen volume or the instructions have not been followed correctly e.g. laying the oral cube on its side or upside down - these are the most likely reasons for the control line failing to appear.
Please read the instructions carefully before beginning the test.
Allow the test device to reach room temperature (15-30°C) prior to testing. Ensure the person carrying out the test does not place anything in their mouth including food, drink, gum, tobacco products for at least 10 minutes prior to collection.
- Remove the collection sponge and test cube from the sealed pouch.
- Tear off the packaging around the collection sponge.
Insert the sponge end of the saliva collector into the mouth. Actively swab the inside of the mouth and tongue to collect oral fluid for a total of 3 minutes (until the sponge becomes fully saturated). Gentle pressing of the sponge between the tongue and teeth will assist saturation. No hard spots should be felt on the sponge when saturated. See step 2.
- Hold the test cube vertically and place the collection sponge into the test cube. Make sure to fit the groove of the collection sponge onto the guide rail inside the test cube. Now press the sponge firmly down. See step 3.
- When you are completely happy that you have collected enough saliva sample firmly press down on the lid till it is tightly sealed. This will allow all of the saliva to be extracted from the collection sponge. See step 4. Note: If the amount of saliva pressed into the test cube is not adequate for testing, collect more saliva by re-inserting the collection sponge in the mouth and follow steps 2, 3 and 4 again.
- Wait for the coloured line(s) to appear on the test device. Read results at 10 minutes. Do not interpret results after 1 hour. See step 5. Note: When carrying out the test the oral cube must remain in a vertical position at all times.
What should I do with the results?
If you obtain a negative result then the person tested has none of the tested drugs of abuse in their body at this time. You may want to re-test again in a month’s time. If you obtain a positive result then a drug of abuse has been detected in the saliva. You may want to do another test later, or at a further date to confirm the result.